Thermo Fisher Scientific TIMP4 Monoclonal Antibody (9:4-7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (IHC)

5-10 µg/mL

-

ELISA (ELISA)

1:20,000-1:100,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

9:4-7

Immunogen

Anti-TIMP4 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with full length human TIMP4 protein followed by hybridoma development. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TIMP4,uniProtId:Q99727-1,ncbiNodeId:9606,antigenRange:1-224,antigenLength:224,antigenImageFileName:200-301-E65_TIMP4_Q99727-1_House_mouse.svg,antigenImageFileNamePDP:200-301-E65_TIMP4_Q99727-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

TIMP4 antibody is specific for human TIMP4 protein. This antibody reacts with human TIMP4 in tissue sections of formalin-fixed, paraffin-embedded In ELISAs and Native-PAGE, this antibody does not cross-react with recombinant human TIMP-1, -2 or -3. A BLAST analysis was used to suggest cross-reactivity with TIMP4 from human and mouse sources based on the immunizing sequence. Cross-reactivity with TIMP-4 from other sources has not been determined.

Target Information

Tissue inhibitor of metalloproteinase 4 (TIMP4) is encoded by the gene TIMP4 located on chromosome 3. It belongs to the family of TIMP genes and is known to inhibit the activity of matrix metalloproteinases. Matrix metalloproteinases (MMP) are a group of proteinases that degrade the components of extracellular matrix. TIMP4 irreversibly binds to MMPs and inactivates them with the help of zinc as a cofactor. The netrin domain of TIMP4 is involved in platelet recruitment and aggregation. Other biological functions include response to cytokine and peptide hormone stimuli and involvement with the ovulation cycle and CNS development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.