Thermo Fisher Scientific KCNJ5 Polyclonal Antibody, Biotin

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 1:50–1:250

Product Specifications

Target Information

G protein-coupled inwardly rectifying potassium channels (Kir3.1 through Kir3.4) are coupled to numerous neurotransmitter receptors in the brain and are abundantly expressed in the olfactory bulb, hippocampus, neocortex, dentate gyrus, cerebellar cortex, and thalamus regions of the brain. Also known as GIRK, Kir3 potassium channels localize to the soma and dendrites as well as axons of neurons. Liberated Gβγ subunits from G protein heterotrimers bind to and regulate Kir3 channel activity. Gβ3- and Gβ4-containing Gβγ dimers bind directly to cytoplasmic domains of Kir3 proteins and increase the K⁺ current, while Gβ5-containing Gβγ dimers inhibit Kir3 K⁺ current. Kir3 activity is also inhibited by tyrosine phosphorylation. Brain-derived neurotrophic factor activates receptor tyrosine kinase B, which then phosphorylates Kir3 tyrosine residues, effectively inactivating the Kir3 channels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.