Thermo Fisher Scientific Baculovirus Envelope gp64 Monoclonal Antibody (AcV1), PE, eBioscience

Applications

Western Blot (WB)

• Not tested

Flow Cytometry (Flow)

• Tested Dilution: 0.125 µg/test

Product Specifications

Additional Formats

• Unconjugated (Cat. 14-6991-82)
• APC conjugate (Cat. 17-6991-80)
• Custom conjugation available upon request

Product Specific Information

The AcV1 monoclonal antibody reacts with the gp64 envelope protein of the baculovirus Autographa californica (AcMNPV).
The gp64 protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection.
This antibody is suitable for flow cytometry-based viral titration experiments and identification of virally infected insect cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometry on baculovirus-infected insect cells
  • Recommended ≤ 0.125 µg per test (100 µL final volume)
  • Suitable cell range: 10⁵–10⁸ cells/test
  • Optimize titration for best performance
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Baculoviruses infect invertebrates, with over 600 species described.
They primarily infect larval moth species but can also infect sawflies, mosquitoes, and shrimp.
Although capable of entering mammalian cells in culture, baculoviruses do not replicate in mammalian or other vertebrate cells.
The gp64 envelope protein is critical for viral infectivity and appears on infected cell surfaces within six hours post-infection.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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