Thermo Fisher Scientific CDK9 Polyclonal Antibody
CDK9 단백질을 인식하는 Rabbit Polyclonal Antibody로 다양한 응용(WB, IHC, ELISA, IP)에 사용 가능. 인간 CDK9의 C- 및 N-말단 도메인 합성 펩타이드로 면역화. 비결합형 액상 형태이며 4°C 단기, -20°C 장기 보관. 세포 성장 및 전사 조절 연구에 적합.
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Thermo Fisher Scientific CDK9 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Reference |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| ELISA | 1:10,000–1:50,000 | - |
| Immunoprecipitation (IP) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene CDK9 (PITALRE) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2077308 |
Product Specific Information
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
- No reaction was observed against other related cyclin-dependent kinases.
Target Information
CDK9 is a serine/threonine kinase that forms an active holoenzyme complex when associated with CyclinT1. This complex plays a crucial role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1 and regulates cytokine-inducible transcription networks by facilitating promoter recognition of target transcription factors.
CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also exhibits kinase activity toward the CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement, leading to cardiac hypertrophy and increased susceptibility to heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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