Thermo Fisher Scientific SMURF1 Monoclonal Antibody (1D7)
SMURF1 단백질을 인식하는 Mouse monoclonal antibody (Clone 1D7). Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합. Human 시료 반응성. PBS buffer, no preservative, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 2 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.1 ng/mL |
| Immunoprecipitation (IP) | 1–5 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D7 |
| Immunogen | SMURF1 (NP_065162, 165 a.a. – approximately 268 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
DSGPGRPLSC FMEEPAPYTD STGAAAGGGN CRFVESPSQD QRLQAQRLRN PDVRGSLQTP QNRPHGHQSP ELPEGYEQRT TVQGQVYFLH TQTGVSTWHD PRIP
Target Information
Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway.
Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3.
Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4.
Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell.
Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controls the local level of RhoA.
Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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