Thermo Fisher Scientific SOX9/SRY-box 9 Monoclonal Antibody (SOX9, 2398)
SOX9 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC(P), Peptide Array 등에 사용 가능. 인간 시료 반응성, 마우스 IgG1 아이소타입, 단백질 A/G 정제. PBS(pH 7.4) 용액 형태로 제공되며 보존제 없음.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SOX9, 2398 |
| Immunogen | Recombinant human SOX9 protein fragment (around aa 393–508) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C or –80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
SOX9 plays a crucial role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and acts as a transcription factor regulating genes associated with cartilage formation.
Translocation of the SOX9 gene can cause campomelic dysplasia. It contributes to the formation of testes from fetal gonads and is a key factor in neuron-glia switching during spinal cord development.
In prostate development, SOX9 supports epithelial outgrowth and basal cell maintenance; in cancer, these functions can promote tumor growth and invasion.
SOX9 also participates in neural crest precursor formation and regulates melanin production. Expression is observed in adult testis, adult heart, and fetal brain by Northern blot analysis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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