Thermo Fisher Scientific SOX9/SRY-box 9 Monoclonal Antibody (SOX9, 2398)
SOX9 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC(P), Peptide Array에 사용 가능. 인간 시료에 반응하며, Protein A/G 정제 및 PBS buffer에 보관. SOX9의 성 결정, 연골 형성, 신경 발달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SOX9, 2398 |
| Immunogen | Recombinant human SOX9 protein fragment (around aa 393–508) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues, heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.
Target Information
SOX9 plays a crucial role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates genes related to cartilage formation by acting as a transcription factor. Translocation of SOX9 can lead to campomelic dysplasia. It is essential for testis formation from fetal gonads and participates in neuron-glia differentiation in the developing spinal cord. SOX9 also influences prostate epithelial development and maintenance, and its dysregulation is associated with prostate cancer progression. Additionally, it contributes to neural crest precursor formation and melanin production regulation. Expression has been observed in adult testis, heart, and fetal brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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