Thermo Fisher Scientific HSF1 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• Immunogen Sequence: DARGHTDTEG RPPSPPPTST PEKCLSVACL DKNELSDHLD AMDSNLDNLQ TMLSSHGFSV DTSALLDLFS PSVTVPDMSL PDLDSSLASI QELLSPQEPP RPPEAENSSP DSGKQLVHYT AQPLFLLDPG SVDTGSNDLP VLFELGEGSY FSEGDGFAED PTISLLTGSE PPKAKDPTVS
• Positive Samples: HeLa, K-562, HepG2, MCF7
• Cellular Location: Cytoplasm, Nucleus

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and are present upstream of all heat shock genes.

HSFs are highly conserved among species, yet HSFs purified from yeast, Drosophila, and human have different molecular weights and show minimal immunological cross-reactivity. Two HSFs have been identified in human cells—HSF1 and HSF2—which bind to the same HSEs and share 38% sequence identity.
HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA binding activity. Under stress, HSF1 becomes activated to a nuclear-localized, trimeric state capable of DNA binding. Phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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