Thermo Fisher Scientific Cas 9 Polyclonal Antibody
Streptococcus pyogenes Cas9 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능. 고순도 Affinity Chromatography로 정제되어 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Cas9 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| ELISA | 1:25,000–1:30,000 |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cas9 affinity purified antibody prepared from whole rabbit serum immunized with a synthetic peptide near the C-terminal of Streptococcus pyogenes Cas9 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas9.
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. It enables precise genome editing by inducing indel mutations, sequence insertions/replacements, or large deletions.
Cas9 variants include:
- SpCas9 (Streptococcus pyogenes)
- SaCas9 (Staphylococcus aureus)
SaCas9 offers advantages such as smaller size, high efficiency, and specificity. It recognizes NNGRRT PAM sequences and performs DNA cleavage with spacer lengths of 20–24 nucleotides, making it suitable for compact vector systems such as AAV delivery.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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