Thermo Fisher Scientific Cyclin D3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

4 µg/mL

-

Flow Cytometry (Flow)

4 µg/mL

-

ELISA (ELISA)

1:1,000-1:5,000

-

Immunoprecipitation (IP)

5 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cyclin D3,uniProtId:P30282-1,ncbiNodeId:10090,antigenRange:292,antigenLength:292,antigenImageFileName:600-401-GN2_Cyclin_D3_P30282-1_Rabbit.svg,antigenImageFileNamePDP:600-401-GN2_Cyclin_D3_P30282-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Cyclin D3 affinity-purified antibody is directed against Cyclin D3 protein. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, and bovine sources based on 100% homology for the immunogen sequence. Cross reactivity with other sources has not been determined. No reactivity was observed against Cyclin D2.

Target Information

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. Diseases associated with CCND3 include Retinoblastoma and Plasma Cell Leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.