Thermo Fisher Scientific Claudin 2 Polyclonal Antibody
Claudin-2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(Paraffin)에서 검증됨. Human과 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 세포 접합부 연구 및 tight junction 단백질 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from C-terminus of human claudin 2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: A431, Skin tissue
- Cellular location: Cellular junction, gap
Target Information
Tight junctions are specialized regions of cell-cell contact abundant in luminal epithelial cell sheets. In electron micrographs, they appear as belt-like bands of sealing strands that encircle each cell. These strands form a “molecular gasket” preventing ions, water, and molecules from leaking between cells, maintaining the barrier and polarity of epithelial tissues.
Peripheral membrane proteins such as ZO-1, ZO-2, ZO-3, cingulin, and symplekin are associated with tight junctions and may play roles in assembly, maintenance, and permeability regulation. Actin filaments also contribute to tight junction regulation.
Claudin-1 and Claudin-2 are ~22 kDa transmembrane proteins forming structural components of tight junction strands. Claudin-2 functions as a paracellular channel with cation (Na⁺) selectivity and is primarily expressed in the liver and kidney, with minor expression in the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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