Thermo Fisher Scientific ERK2 Polyclonal Antibody
ERK2 단백질의 C-말단 합성 펩타이드로부터 유래한 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태. 연구용으로만 사용 가능.
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Thermo Fisher Scientific ERK2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 2–10 µg/mL |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of ERK2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: A431 cells
- Cellular location: Cytoplasmic
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway.
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development.
The activation of this kinase requires phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of stimulated cells, where it phosphorylates nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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