Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio64DEC17), eFluor 450, eBioscience

Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio64DEC17), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 605
• Brilliant Violet 786
• eFluor 506
• eFluor 660
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The eBio64DEC17 antibody reacts with human IL-17A and functions as a neutralizing antibody.
IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory conditions such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.

Human IL-17A exists as glycosylated 20–30 kDa homodimers and enhances expression of ICAM-1 in fibroblasts. It stimulates epithelial, endothelial, and fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2, supporting hematopoietic progenitor proliferation and neutrophil maturation.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th17 cells) represent a distinct subset of helper T cells, negatively regulated by IFN-γ and IL-4.
Intracellular staining by eBio64DEC17 identifies the same population as eBio64CAP17.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated for intracellular staining and flow cytometric analysis of stimulated human peripheral blood or Th17 polarized cells.
Use at 5 µL (0.5 µg) per test (final volume 100 µL). Suitable for 10⁵–10⁸ cells/test.

Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited by Violet laser (405 nm), emitting at 445 nm.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in several autoimmune diseases.
IL-17A is a 32 kDa disulfide-linked homodimer of 136 amino acids, signaling via IL-17R (CDw217).
It regulates NF-κB and MAPK activity, induces IL-6 and COX-2 expression, and enhances nitric oxide production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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