Thermo Fisher Scientific IL-21 Monoclonal Antibody (FFA21), eFluor 450, eBioscience
마우스 IL-21을 인식하는 단클론 항체로, eFluor 450 형광체로 결합되어 유세포분석에 적합합니다. Th17 및 TFH 세포 내 IL-21 발현 분석에 활용됩니다. 405/445 nm 파장에서 여기/방출되며, 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Intracellular) | ≤ 1.0 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FFA21 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2811832 |
Available Formats
Product Specific Information
This FFA21 monoclonal antibody reacts with mouse interleukin-21 (IL-21), a 17 kDa immunomodulatory cytokine mainly produced by NKT, Th17, and TFH cells.
In TFH cells, IL-21 expression triggers autocrine signaling through IL-21R and STAT3, activating Bcl6 transcription. IL-21 is essential for TFH effector function, B cell differentiation into plasma and memory cells, IgG production, and apoptosis induction in naive B cells.
In Th17 cells, IL-21 signaling through STAT3, IRF4, and RORγt upregulates IL-23R, supporting Th17 maturation and maintenance. IL-21 also downregulates Foxp3. Elevated IL-21 levels are observed in colitis models, whereas IL-21-deficient mice are resistant to chemically induced colitis.
Preliminary data suggest that clone FFA21 recognizes a different epitope than anti-mouse IL-21 clone mhalx21 (Product #51-7213-83).
Applications Reported: Suitable for intracellular staining and flow cytometric analysis.
Applications Tested: Validated using Th17-polarized mouse splenocytes restimulated with PMA, ionomycin, and brefeldin A for 5 hours, following the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) and standard protocol available at www.thermofisher.com/flowprotocols.
Recommended usage: ≤ 1.0 µg per test (100 µL final volume; 10⁵–10⁸ cells/test). Optimize concentration for best performance.
Fluorochrome Information:
eFluor 450 is an alternative to Pacific Blue™, excited by the violet laser (405 nm) and emitting at 446 nm. Ensure instrument compatibility.
Target Information
IL-21 is a 17 kDa cytokine produced by NKT, Th17, and TFH cells. It mediates autocrine signaling via IL-21R and STAT3, promoting Bcl6 activation and TFH cell function. IL-21 regulates B cell differentiation, IgG production, and apoptosis in naive B cells.
In Th17 cells, IL-21 enhances IL-23R expression via STAT3, IRF4, and RORγt, while suppressing Foxp3. IL-21-deficient mice show resistance to colitis, whereas elevated IL-21 levels are found in inflammatory models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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