Thermo Fisher Scientific BRD7 Polyclonal Antibody
BRD7 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, Flow cytometry, IP 등 다양한 응용에 적합. 높은 특이성과 항원 친화 정제 방식으로 제조되어 신뢰성 높은 연구 결과 제공. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 0.5 µL per 1 x 10^6 cells |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 601 and 651 of human bromodomain containing 7 (BRD7, UniProt ID: Q9NPI1-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
BRD7 acts both as coactivator and corepressor. It activates the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating GSK3B phosphotransferase activity. It is also required for TP53-mediated cell-cycle arrest in response to oncogene activation. BRD7 promotes acetylation of TP53 at Lys-382, enhancing recruitment of TP53 to target promoters and inhibiting cell cycle progression from G1 to S phase.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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