Thermo Fisher Scientific Phospho-IRS-1 (Ser312) Polyclonal Antibody
인슐린 수용체 기질 단백질 IRS-1의 Ser312 인산화 형태를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot과 IHC(파라핀) 검증 완료. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the epitope ATSPA with a single phosphorylation site Ser312 of human IRS-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: CHO cell lysate, human breast carcinoma, and cervical carcinoma tissues
- Cellular location: Cytosol and nucleus
Target Information
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation by insulin, IGF-1, and IL-4. It undergoes phosphorylation on serine, threonine, and tyrosine residues in various tissues. Casein kinase II mediates part of the insulin-stimulated phosphorylation of IRS-1, with Thr502 and Ser99 identified as major phosphorylation sites. IRS-1 also contains three putative 14-3-3 binding sites (Ser270, Ser374, Ser641), with the Ser270 motif located in the phosphotyrosine binding domain responsible for insulin receptor interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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