Thermo Fisher Scientific Phospho-Pdcd4 (Ser457) Monoclonal Antibody (9G6)
Phospho-Pdcd4 (Ser457) 단백질을 특이적으로 인식하는 mouse monoclonal antibody. Western blot, IHC, ELISA, IP 등 다양한 응용 가능. Human, mouse, rat, Xenopus 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Thermo Fisher Scientific Phospho-Pdcd4 (Ser457) Monoclonal Antibody (9G6)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (IHC) | 1.25–2.5 µg/mL |
| ELISA | 1:400,000 |
| Immunoprecipitation (IP) | 1:1,000–1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Xenopus |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9G6 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ser457 of human Pdcd4 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. BLAST analysis suggests cross-reactivity with Pdcd4 from human, mouse, rat, and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
Target Information
Apoptosis (programmed cell death) plays major roles in development, tissue turnover, and tumor formation. PDCD4 encodes a tumor suppressor protein whose expression is lost in several carcinomas (breast, colon, lung, prostate).
PDCD4 can bind and inhibit the helicase activity of eIF4A and suppress AP-1 mediated transactivation. Akt-mediated phosphorylation of PDCD4 decreases its inhibitory effect on c-Jun transactivation.
Two isoforms of PDCD4 are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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