Thermo Fisher Scientific LGI1 Monoclonal Antibody (N283/7), APC
LGI1 단백질을 인식하는 APC 결합 마우스 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 단백질 G로 정제된 액상 제품이며, 형광 분석용으로 적합. 4°C 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N283/7 |
| Immunogen | Fusion protein amino acids 37–113 (LRRNT domain and first LRR repeat) of mouse LGI1 |
| Conjugate | APC (Allophycocyanin) |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932131 |
Additional Formats Available
- Unconjugated (MA5-27652)
- FITC (MA5-45678)
- PE (MA5-45680)
- PerCP (MA5-45679)
- Custom conjugation available upon request
Product Specific Information
- Rat: 100% identity (77/77 amino acids identical)
- Human: 98% identity (76/77 amino acids identical)
- Approximately 50% identity with LGI2, LGI3, and LGI4
- 1 µg/mL of MA5-45677 detects LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP as secondary antibody
- Detects approximately 60 kDa
- Formerly sold as clone S283-7
Target Information
The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 belongs to a family of proteins containing leucine-rich repeats (LRRs), similar to other transmembrane signaling molecules and receptors. It acts as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 are associated with autosomal dominant lateral temporal epilepsy (ADTLE), characterized by focal seizures with auditory symptoms. Two isoforms of LGI1 are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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