Thermo Fisher Scientific VprBP Polyclonal Antibody
Rabbit polyclonal antibody recognizing Human VprBP. Validated for Western blot and immunoprecipitation. Affinity purified, supplied as liquid in TBS buffer with BSA and sodium azide. Recommended storage at 4°C, for research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1457 and 1507 of Human Vpr (HIV-1) binding protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
VprBP acts as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase. It plays key roles in processes such as cell cycle regulation, telomerase control, and histone modification.
It is part of the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, mediating ubiquitination and degradation of target proteins like NF2.
VprBP recognizes methylated proteins via its chromo domain, contributing to protein turnover and transcriptional repression through phosphorylation of histone H2A (Thr-120).
It is also implicated in B-cell development, JNK-mediated apoptosis, and viral infection mechanisms of HIV-1 and HIV-2, where viral proteins hijack the complex to manipulate host cell cycle and degradation pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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