Thermo Fisher Scientific IFN gamma Polyclonal Antibody
고양이 IFN-γ를 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot과 중화반응에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 보존제 없이 동결건조 형태로 제공. 사람, 생쥐, 쥐, 돼지 IFN-γ와의 교차반응이 1% 미만.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Neutralization (Neu) | 1–3 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Cat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant feline IFN-gamma (Gln24–Lys167) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608739 |
Product Specific Information
- In direct ELISAs, less than 1% cross-reactivity with human, mouse, rat, and porcine IFN-gamma is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and natural killer cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. On SDS-PAGE, it appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Its functions include:
- Antiviral and tumor antiproliferative activity
- Induction of MHC class I and II
- Macrophage activation
- Enhancement of immunoglobulin secretion by B lymphocytes
IFN gamma activity is highly species-specific; human IFN gamma does not cross-react with mouse. It acts via binding to IFN gamma receptor I and II, activating the JAK-STAT pathway. Expression is upregulated by IL-2, FGF basic, and EGF, and downregulated by vitamin D3 or DMN. Mutations in the IFN gamma gene are associated with aplastic anemia.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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