Thermo Fisher Scientific Cre recombinase Polyclonal Antibody
Cre recombinase 단백질을 인식하는 토끼 폴리클로날 항체로, WB, IHC, IP 등에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 제품. 바이러스 반응성, 비결합형 액상 형태. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Cre recombinase Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Virus |
| Published Species | Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549718 |
Product Specific Information
- Recommended positive controls: 293T transfected recombinant Cre protein, IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cre recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA.
It catalyzes site-specific recombination between two 34-base-pair loxP sites.
Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
It forms a homotetramer when bound to DNA and belongs to the phage integrase family.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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