Thermo Fisher Scientific Influenza A NP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 4 publications 4 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment of Influenza A virus NP (A/WSN/1933(H1N1))

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549715

Product Specific Information

Recommended positive controls: H1N1-infected H1299, DF1 (Influenza A virus treatment for 10hr), influenza virus (A/WSN/33) infected MDCK.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The nucleoprotein (NP) of Influenza A virus encapsidates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56 (Portela et al 2002).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.