Thermo Fisher Scientific HMGB1 Polyclonal Antibody
Thermo Fisher Scientific의 HMGB1 Polyclonal Antibody는 인간, 마우스, 랫트에 반응하는 Rabbit IgG 항체로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도와 안정성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 1–215 of Human HMGB1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.17 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549722 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat brain
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA-binding chromosomal protein. Recent studies indicate that damaged or necrotic cells release HMGB1 extracellularly, where it functions as a proinflammatory cytokine.
Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains: two DNA-binding domains (HMG box A, aa 9–79; box B, aa 89–162) and a negatively charged C-terminal acidic tail (30 aa). Nuclear localization signals are located at residues 28–44 and 180–185.
The cytokine activity is associated with the B box, while the A box interacts with transcription factors. HMGB1 promotes monocyte migration, cytokine secretion, and T cell–dendritic cell interactions. It signals through receptors such as RAGE, TLR2, and TLR4.
HMGB1 is highly conserved and ubiquitous in nuclei and cytoplasm, serving as a key mediator of inflammation during sterile and infection-associated responses. Elevated HMGB1 levels are associated with tissue injury, sepsis, arthritis, and ischemia-reperfusion injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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