Thermo Fisher Scientific beta Tubulin Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Keep as concentrated solution.
• Predicted reactivity: Xenopus laevis (99%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
• Positive Control: 293T, A431, HeLa, HepG2, A549, H1299, HCT116, NIH-3T3, MCF7, Huh7.
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene are associated with congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin has also been detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, though the precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, which inhibits its incorporation into microtubules.

Microtubules, composed of alpha and beta tubulin dimers (~55 kDa each), are essential cytoskeletal elements involved in chromosome segregation, intracellular transport, ciliary and flagellar movement, and structural support. Because beta-tubulin is ubiquitously expressed in eukaryotic cells, it is frequently used as a loading control in protein detection assays such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.