Thermo Fisher Scientific Adenylate Kinase 1 Polyclonal Antibody
Rabbit polyclonal antibody against Adenylate Kinase 1 (AK1), reactive with human and mouse samples. Suitable for WB, IHC, ICC, and ELISA applications. Affinity purified, liquid form, stored at -20°C with glycerol for stability.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–194 of human AK1 (NP_0004671) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.23 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804885 |
Product Specific Information
Immunogen sequence:
MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK
Positive Samples: Mouse heart, Mouse skeletal muscle
Cellular Location: Cytoplasm
Target Information
Adenylate kinase regulates adenine nucleotide composition within cells by catalyzing reversible phosphate transfer among adenosine nucleotides. Three isozymes—AK1, AK2, and AK3—are found in vertebrates.
- AK1: Cytosolic, present in skeletal muscle, brain, and erythrocytes
- AK2 & AK3: Mitochondrial, found in liver and heart tissues
AK1 mutations are associated with nonspherocytic hemolytic anemia due to reduced catalytic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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