Norgen HighRanger Plus 100 bp DNA Ladder

Norgen HighRanger Plus 100 bp DNA Ladder

SKU: 12000
Ideal for size determination of digested DNA.

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Features and Benefits

• Ready-to-use
• Quantitative
• Highly stable
• Precise sizing
• Nineteen discrete fragments ranging from 100 bp to 10,000 bp
• Higher intensity reference bands at 500 bp, 1000 bp, and 5000 bp

The Norgen HighRanger Plus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. It contains nineteen discrete fragments ranging from 100 bp to 10,000 bp with higher intensity reference bands at 500 bp, 1000 bp, and 5000 bp. This ladder is ideal for size determination of digested DNA.

Contents:
1 mL of premixed DNA ladder (0.5 µg/10 µL) in loading buffer (10 mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

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Ladder Properties

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Recommended Use

Mix thoroughly before use. For best results, load 10 µL of DNA ladder per well.
For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5 µg/mL ethidium bromide for 30–40 minutes.
The table above shows the size and mass for each band based on 10 µL ladder per well.

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Storage

Stable for 2 years after the date of shipment.
Standardized using 10 µL of DNA per lane on a 0.8 cm thick, 13 × 15 cm, 1.0% agarose gel run in TAE buffer.

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Supporting Data

Figure 1: HighRanger Plus 100 bp DNA Ladder

표: DNA Ladder 정보 (이미지 OCR 추출 텍스트)

“10 µL on 1% agarose”

이 표는 DNA ladder의 밴드 크기(bp)와 각 밴드의 농도(ng/10µL)를 나타냅니다.

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Documentation

• Product Insert (Cat# 12000)
• Application Note: DNA Quantification by Gel Densitometry with Norgen DNA Ladders
• Safety Data Sheet (SDS)

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Citations

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