Thermo Fisher Scientific DNAJB2 Monoclonal Antibody (OTI5C11), TrueMAB
DNAJB2 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 유세포분석에 적합. 인간 시료 반응성, 동결건조 형태로 제공되며, 고순도 친화 크로마토그래피 정제. 연구용으로 뇌 조직 내 DNAJB2 발현 연구에 활용 가능.
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Thermo Fisher Scientific DNAJB2 Monoclonal Antibody (OTI5C11), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C11 |
| Immunogen | Full length human recombinant protein of human DNAJB2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve an antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting column: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
The DnaJ family comprises a large group of chaperone proteins with diverse cellular localizations and functions. The J domain defines membership in this family.
DnaJ proteins, regulated by the htpR protein in E. coli, interact with HSP70 to stimulate ATP hydrolysis and contain cysteine-rich zinc finger regions forming peptide-binding domains for chaperone activity.
These proteins are key mediators of proteolysis, protein degradation regulation, exocytosis, and endocytosis.
DNAJB2 (DnaJ homolog subfamily B member 2), also known as HSJ1 or HSPF3, is predominantly expressed in the brain, particularly in the frontal cortex and hippocampus. Two isoforms are produced via alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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