Thermo Fisher Scientific DES Monoclonal Antibody (OTI4G1), TrueMAB
인간 Desmin 단백질을 인식하는 마우스 단클론 항체로, WB, IHC(P), Flow Cytometry에 적합합니다. 무보존제 동결건조 형태이며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 근육세포 특이적 단백질 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4G1 |
| Immunogen | Human fragment corresponding to amino acids 104–391 of human DES produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7 K MWCO, 0.5 mL, Product #89882).
Target Information
Desmin (~53 kDa) is a muscle-specific intermediate filament protein predominantly expressed in cardiac, skeletal, and smooth muscle cells. Its expression is regulated in a stage- and tissue-specific manner, being induced during terminal muscle cell differentiation.
In skeletal and cardiac muscle, desmin localizes to the Z-disk and intercalated disk regions. In smooth muscle, expression is more heterogeneous. Coexpression of vimentin and desmin is often observed in muscle-derived tumors such as rhabdomyosarcomas and leiomyosarcomas. Altered desmin distribution has been associated with myocardial dysfunction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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