Thermo Fisher Scientific O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (9D1.E4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

5 µg

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Product Specifications

Species Reactivity

Chemical

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

9D1.E4

Immunogen

SANM-conjugated linear peptide corresponding to human O-GluNAc.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536726

Product Specific Information

MA1-039 has been successfully used in Western blot and immunoprecipitation applications with human samples. In Western blot, MA1-039 detects proteins with O-GlcNAc post-translational modification, and therefore will detect multiple specific bands.

Target Information

O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues. Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm. They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins. O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.