Thermo Fisher Scientific PSD-95 Monoclonal Antibody (6G6-1C9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 91 publications 91 publications

Immunohistochemistry (IHC)

-

View 60 publications 60 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:500

View 104 publications 104 publications

Flow Cytometry (Flow)

2 µg/test

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 9 publications 9 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

Immunomicroscopy (IM)

-

View 1 publication 1 publication

GST Pull Down (GPD)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

C. elegans, Human, Mouse, Nematode, Non-human primate, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6G6-1C9

Immunogen

Purified recombinant rat PSD-95.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.3 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325399

Product Specific Information

MA1-045 has been successfully used in immunofluorescence analysis of PSD95 in human motor neurons derived from iPSCs.

Target Information

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.