Thermo Fisher Scientific IkB alpha Monoclonal Antibody (F.4.4)
IkB alpha 단백질을 표적으로 하는 Thermo Fisher Scientific의 단클론 항체로, Western blot과 Immunoprecipitation에 적합합니다. Mouse IgG2a 형식이며, 다양한 종(Human, Mouse 등)에 반응합니다. 액상 형태로 제공되며 -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | F.4.4 |
| Immunogen | Synthetic peptide corresponding to residues near the carboxy-terminus of human IkB alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 871.7 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10978164 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
IkB-alpha is a 40 kDa protein that functions to inhibit NF-kappaB activity. The inhibition occurs via protein-protein interaction between IkB proteins and NF-kappaB dimers in the cytosol. The interaction of IkB-alpha with NF-kappaB masks the nuclear localization sequence of NF-kappaB, preventing its translocation to the nucleus. Various stimuli such as TNF-alpha, IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, and other stresses can lead to degradation of IkB-alpha, thereby activating NF-kappaB.
In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires phosphorylation of IkB on specific serine residues (Ser32 and Ser36), leading to its degradation. IkB kinase alpha (IKK alpha, also known as CHUK) interacts with and phosphorylates IkB-alpha at these sites. IKK alpha is critical for NFkB activation in response to proinflammatory cytokines and is regulated by the NFkB inducing kinase (NIK). The functional IKK complex consists of IKK alpha, IKK beta, and IKK gamma, each contributing to IkB phosphorylation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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