Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), eFluor 450, eBioscience
인간 Glycophorin A(CD235a)을 인식하는 HIR2 단클론 항체로, eFluor 450 형광체로 결합됨. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기되어 445 nm에서 방출. 적혈구 및 전구세포 표지에 적합.
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Applications
Immunocytochemistry (ICC/IF)
- Not specified
Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.03 µg)/test
- View 28 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIR2 (GA-R2) |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2574140 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.
This antibody is reported for use in flow cytometric analysis. It has been pre-titrated and tested by flow cytometric analysis of diluted normal human peripheral blood cells.
Binding of this antibody to red cells at high antibody concentration causes cell agglutination. Recommended usage is 5 µL (0.03 µg) per test, with a final volume of 100 µL. Cell number may range from 10⁵ to 10⁸ cells/test.
eFluor™ 450 is an alternative to Pacific Blue™, emitting at 445 nm when excited at 405 nm (Violet laser). Ensure that the instrument is compatible with this fluorochrome.
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD235a (Glycophorin A, GPA) is a single-pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells.
It carries blood group M and N specificities and provides a mucin-like barrier that minimizes red blood cell aggregation.
CD235a also acts as a receptor for Sandei virus, parvovirus, and Hsa, a Streptococcus adhesin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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