Thermo Fisher Scientific Human PLK1, His Tag Recombinant Protein
Human PLK1 His-tag 재조합 단백질로, 세포 분열 및 암 발생 연구에 활용 가능. Insect cell에서 발현된 full-length 단백질이며, 85% 이상 순도 확보. Kinase assay 등 연구용으로 적합하며, -80°C 보관 필요.
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human PLK1, His Tag Recombinant Protein
Applications
- Kinase Assay (KA): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Insect cells |
| Amino Acid Sequence | Full length |
| Tag | His-tag |
| Molecular Weight | 73.3 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | 85% by SDS-PAGE |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 20mM HEPES, pH 7.5, with 0.05% Triton X-100, 50% glycerol, 5mM DTT, 0.5mM EDTA, 150mM NaCl, 0.5mM EGTA |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
- For maximum recovery, please spin prior to use.
- Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use materials in original packaging until exhausted.
- For larger sizes, reduce freeze/thaw cycles by preparing aliquots (do not prepare aliquots below 20 µL).
- Never store kinase diluted.
- Properly stored at -80°C, product guaranteed for 6 months from date of purchase.
- Protein Form: Full Length
Target Information
PLK1 is a serine/threonine kinase involved in cell mitosis and is considered a marker of cell proliferation. It plays a critical role in carcinogenesis. Polo-like kinase proteins bind and phosphorylate proteins already phosphorylated on motifs recognized by POLO box domains.
PLK1 phosphorylates numerous substrates including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, and WEE1.
It plays a key role in centrosome function and bipolar spindle assembly by phosphorylating KIZ, NEDD1, and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.