Thermo Fisher Scientific V5 Epitope Tag Polyclonal Antibody, DyLight 488
DyLight 488 형광 표지를 가진 V5 epitope tag 인식 polyclonal antibody로, Western blot, ICC/IF, ELISA 등에 사용 가능. 친화 정제된 rabbit IgG 항체로 높은 특이성과 감도를 제공. 연구용 단백질 발현 검출 및 위치 확인에 적합.
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Thermo Fisher Scientific V5 Epitope Tag Polyclonal Antibody, DyLight 488
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 95–108 (G-K-P-I-P-N-P-L-L-G-L-D-S-T) of the V protein conjugated to KLH using maleimide |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; -20°C long term (avoid freeze/thaw) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This affinity purified antibody is directed against the V5 epitope tag and is useful in detecting its presence in overexpressed proteins across various assays. It recognizes the V5 epitope tag (Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr) fused to either amino- or carboxy-termini of target proteins in transfected or transformed cells.
Target Information
Epitope tags allow localization of gene products in various cell types, study of protein topology and complexes, identification of associated proteins, and characterization of low-abundance or poorly immunogenic proteins when specific antibodies are unavailable.
The V5 epitope tag originates from the P and V proteins of the paramyxovirus of simian virus (SV5). It typically uses the full 14 amino acid sequence (GKPIPNPLLGLDST), but shorter versions (IPNPLLGLD) are also used.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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