Thermo Fisher Scientific DYKDDDDK Polyclonal Antibody, DyLight 488
DYKDDDDK(FLAG) 태그를 인식하는 DyLight 488 결합 폴리클로날 항체로, Western blot, ICC/IF, ELISA 등 다양한 단백질 검출에 사용 가능. 고순도 정제된 Rabbit IgG로 높은 특이성과 형광 감도를 제공. 연구용 단백질 발현 및 태그 검증에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | 1:10,000–1:25,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified from rabbit serum immunized with Enterokinase Cleavage Site (ECS) peptide (D-Y-K-D-D-D-D-K) conjugated to KLH using maleimide. Reacts with FLAG™ conjugated proteins. |
| Conjugate | DyLight™ 488 |
| Excitation/Emission Max | 492 / 519 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This affinity purified antibody specifically recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy-terminus of target proteins. Useful for detecting FLAG-tagged proteins in transfected or transformed cells across various assays.
Target Information
DYKDDDDK tag (FLAG tag) is an eight-amino-acid peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) used as a protein tag for purification, detection, and localization. It can be fused to the N- or C-terminus of proteins, allowing easy identification and isolation using anti-FLAG antibodies.
Widely used in molecular biology and biochemistry for:
- Protein-protein interaction studies
- Protein expression analysis
- Protein localization studies
The FLAG tag is hydrophilic, minimizing interference with protein folding or function.
FLAG™ is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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