
Thermo Fisher Scientific Goat anti-Human IgG (Heavy chain) Secondary Antibody
인간 IgG의 헤비체인에 특이적인 염소 유래 2차 항체로, WB, IHC, ICC, ELISA, ISH 등 다양한 응용에 사용 가능. 고순도 친화 크로마토그래피 정제, 낮은 교차 반응성, 안정적인 액상 형태로 장기 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | Assay-dependent |
| In Situ Hybridization (ISH) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 20 mg/mL sucrose |
| Contains | 0.08% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient |
| RRID | AB_228261 |
| Target | IgG (Heavy chain) |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #31118 has been successfully used in Western blot, IF, ICC, IHC, ELISA, and ISH applications.
This antibody reacts with the heavy chains of human IgG, but does not react against non-immunoglobulin serum proteins.
Based on solid-phase enzyme immunoassay, it shows minimal cross-reactivity with human IgE and IgM.
Note that it may cross-react with immunoglobulins from other species.
Activity measured by a solid-phase binding assay is 91%.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins.
They are useful in detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility by enabling use of multiple detection systems (HRP, AP, fluorescence) and increase sensitivity through signal amplification.
These antibodies are typically generated by immunizing host animals with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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