Thermo Fisher Scientific Goat anti-Human IgG (Heavy chain) Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

ELISA (ELISA)

Assay-dependent

In Situ Hybridization (ISH)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.8, with 20mg/mL sucrose

Contains

0.08% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient

RRID

AB_228261

Target

IgG (Heavy chain)

Antibody Form

Whole Antibody

Product Specific Information

Product # 31118 has been successfully used in Western blot, IF, ICC, IHC, ELISA, and ISH applications.

Product # 31118 reacts with the heavy chains of human IgG, but does not react against non-immunoglobulin serum proteins. Based on solid-phase enzyme immunoassay, this antibody has minimal cross-reactivity with human IgE and human IgM. However, this antibody may cross-react with immunoglobulins from other species.

The activity measured by a solid phase binding assay is 91%.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.