
Thermo Fisher Scientific Goat anti-Human Kappa Light Chain Secondary Antibody
Thermo Fisher Scientific의 염소 유래 항-인간 카파 라이트 체인 2차 항체로, WB, IHC, ICC, ELISA, ISH 등 다양한 실험에 사용 가능. 인간 IgG의 카파 라이트 체인에 특이적이며, 낮은 교차반응성을 보임. 액상 형태로 제공되며 4°C 또는 -20°C에서 안정적으로 보관 가능.
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Thermo Fisher Scientific Goat anti-Human Kappa Light Chain Secondary Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | Assay-dependent |
| In Situ Hybridization (ISH) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient |
| RRID | AB_228283 |
| Target | Kappa light chain |
| Antibody Form | Whole Antibody |
Product Specific Information
- Product #31129 has been successfully used in Western blot, IF, ICC, ISH, ELISA, and IHC applications.
- Reacts with the kappa light chains of human IgG, but does not react against non-immunoglobulin serum proteins.
- Minimal cross-reactivity observed: human gamma chain (1%), human lambda chain, and human lambda IgG.
- May cross-react with immunoglobulins from other species.
- Activity measured by solid phase binding assay: 97%.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of specific targets.
Secondary antibodies offer increased versatility and sensitivity through signal amplification, enabling use with various detection systems (HRP, AP, fluorescence).
Typically generated by immunizing the host animal with pooled immunoglobulins from the target species, and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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