Thermo Fisher Scientific Goat anti-Human IgM Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

ELISA (ELISA)

Assay-dependent

In Situ Hybridization (ISH)

Assay-dependent

Product Specifications

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient

RRID

AB_228277

Target

IgM

Antibody Form

Whole Antibody

Product Specific Information

Product # 31124 has been successfully used in Western blot, IF, ICC, IHC, ELISA, and ISH applications.

The activity measured by a solid phase binding assay is 91%.

Product # 31124 reacts with the µ chains of human IgM. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with dog IgM (12%) and mouse IgM (12%). It has minimum cross-reactivity with immunoglobulins from other species.

Reconstitute with 0.5 mL of distilled water (1.0 mg/mL after restoration).

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.