Thermo Fisher Scientific CD83 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.1 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 2.5 µg per million cells

Product Specifications

Product Specific Information

• In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse CD83 is observed.
• Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

CD83 cell surface antigen is a 40–45 kDa glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express low levels of CD83 after stimulation with agents such as Con A or PHA.
In immunohistology, CD83 is strongly expressed by interfollicular interdigitating reticulum cells and weakly by cells within germinal centers. It is also expressed by Langerhans cells in the skin.

The CD83 antigen is a 186-amino-acid single-chain glycoprotein, a member of the immunoglobulin superfamily, composed of:

• Extracellular V-type Ig-like single domain
• Transmembrane region
• Short 40-amino-acid cytoplasmic tail

CD83 undergoes extensive post-translational glycosylation, resulting in a molecular weight approximately twice that of its core protein. CD83+ cells have a unique surface immunophenotype distinct from T, B, NK, or myelomonocytic cells, and coexpress high levels of MHC class II molecules along with various lineage markers (CD2, CD5, CD40, CD78, CD13, CD33, CD36) and cytokine receptors.
Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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