Thermo Fisher Scientific Human Caspase 8 Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 15 amino acids near the carboxy terminus of human Caspase-8 isoform E.
PEP-0237 can be used as a blocking peptide with polyclonal antibody PA5-20119.

Target Information

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits.

Caspase 8 is involved in programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests interaction with Fas-interacting protein FADD. It has been detected in the insoluble fraction of affected brain regions from Huntington disease patients, suggesting a role in neurodegenerative diseases.

Caspase 8 binds to the death effector domain (DED) of FADD through a tandem DED domain in its pro-form. Activated Caspase 8 then activates downstream caspases, including Caspase 9, committing the cell to undergo apoptosis. It also reacts with Jurkat cells and tonsil tissues. Overexpression induces apoptosis, which can be blocked by ICE family inhibitors. Multiple alternatively spliced transcript variants exist, though not all have been fully characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.