Thermo Fisher Scientific CD11c Monoclonal Antibody (118/A5), Biotin, eBioscience
휴먼 CD11c를 인식하는 단클론 항체로, Biotin으로 표지되어 있습니다. 동결 및 포르말린 고정 조직의 면역조직화학(IHC) 분석에 적합하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 친화 크로마토그래피로 정제되었으며, 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 118/A5 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572827 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
Product Specific Information
Description:
Monoclonal antibody 118/A5 recognizes human CD11c, a 150 kDa type I transmembrane protein also known as integrin alpha X. Integrins are heterodimeric membrane proteins composed of alpha and beta chains involved in cell adhesion. The CD11/CD18 adhesion molecules share a common beta subunit (CD18) with distinct alpha subunits (CD11a, CD11b, CD11c).
CD11c/CD18 complex is expressed on dendritic cells (especially type 1 myeloid DCs), monocytes, macrophages, granulocytes, NK cells, and subsets of T and B cells. CD11c is also found in hairy cell leukemia, acute nonlymphocytic leukemia, and some B-cell chronic lymphocytic leukemias.
CD11c participates in adhesion and cytotoxic T lymphocyte (CTL) killing via interactions with CD54, iC3b, and fibrinogen.
Applications Reported:
Suitable for immunohistochemical staining of frozen and formalin-fixed paraffin-embedded tissue sections, as well as microscopy.
Applications Tested:
Tested by immunohistochemistry on formalin-fixed paraffin-embedded human tissue using low pH antigen retrieval. Recommended concentration ≤ 5 µg/mL. Optimal dilution should be determined experimentally.
Target Information
CD11, together with CD18, forms a heterodimeric adhesion molecule.
- CD11a: Expressed on B and T lymphocytes, macrophages, monocytes, neutrophils, basophils, and eosinophils.
- CD11b: Found on monocytes, macrophages, NK cells, some B/T cells, and granulocytes.
- CD11c: Expressed on monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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