Thermo Fisher Scientific Mouse CCL5 (RANTES) (Carrier-Free) Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Product Specifications

Expression System

E. coli

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C

Shipping conditions

Dry ice

Product Specific Information

Description: Mouse CCL5, or RANTES (regulated on activation normal T cell expressed and secreted), is an 8 kDa monomeric protein that belongs to the CC- family of chemokines. The chemokines in this family share a common g-protein coupled receptor, CCR5, which they bind to through a conserved core region. In humans, CCR5 is also known to be the co-receptor through which the HIV virus enters the cell. This effect is blocked by the binding of RANTES to CCR5, making these proteins potential targets for HIV therapy.

RANTES plays an important role in the immune response by initiating the recruitment of leukocytes to the site of inflammation. It is a chemoattractant for T cells, monocytes, and dendritic cells, and may increase the cytotoxicity and promote proliferation of NK cells. It is reported to be produced by fibroblasts, platelets, and T cells. RANTES is also involved in the progression and metastasis of some forms of cancer, and constitutive expression has been demonstrated in some malignant tumors.

Human and mouse RANTES share 85% sequence homology and demonstrate cross-species activity.

Applications Reported: Recombinant mouse CCL5 (RANTES) is biologically active and can promote chemoattraction of mouse T cells in culture.

Applications Tested: This protein has been demonstrated to chemoattract murine T cells when used at 1.0-10.0 ng/mL.

Source: E. coli (amino acids Ser24-Ser91; accession # NP_038681).

Bioactivity: This protein has been demonstrated to chemoattract murine T cells when used at 1.0-10.0 ng/mL.

Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC.

Molecular Weight: 7.8 kDa.

Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier protein (e.g., 0.1% BSA) at less than or equal to -20°C.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.