Thermo Fisher Scientific CD44 Monoclonal Antibody (156-3C11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 48 publications 48 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:200

View 6 publications 6 publications

Flow Cytometry (Flow)

2 µg/test

View 7 publications 7 publications

Immunoprecipitation (IP)

2 µg/mL

-

in situ PLA (PLA)

-

View 1 publication 1 publication

Inhibition Assays (IA)

-

View 1 publication 1 publication

Blocking Assay (BLOCK)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Non-human primate, Baboon

Published species

Human, Rabbit, Rhesus monkey

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

156-3C11

Immunogen

Stimulated human leukocytes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD44,uniProtId:P16070-1,ncbiNodeId:9606,antigenRange:1-742,antigenLength:742,antigenImageFileName:MA5-13890_CD44_P16070-1_House_mouse.svg,antigenImageFileNamePDP:MA5-13890_CD44_P16070-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986810

Product Specific Information

MA5-13890 targets CD44 in FACS, IHC (P), ICC/IF, IP and WB applications and shows reactivity with Human, Baboon and Non-human primate samples.

The MA5-13890 immunogen is stimulated human leukocytes.

MA5-13890 was used in the immunofluorescence analysis to successfully detect CD44 in human mesenchymal stem cells derived from iPSCs.

Target Information

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.