Thermo Fisher Scientific POLB Monoclonal Antibody (18S)
POLB 단백질을 인식하는 Thermo Fisher Scientific의 Mouse IgG1 단클론 항체(클론 18S). Western Blot, IP 등 다양한 응용에 적합. Rat DNA Polymerase β를 면역원으로 사용하며, Human, Mouse 등 여러 종에서 반응성 확인. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 11 publications |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| Immunoprecipitation (IP) | 2 µg/mL | View publications |
| ChIP assay (ChIP) | – | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Hamster, Human, Mouse, Rat, Xenopus |
| Published Species | Human, Mouse, Rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 18S |
| Immunogen | Rat DNA polymerase beta protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10977449 |
Product Specific Information
MA5-13899 targets Rat DNA Polymerase β in IP and WB applications and shows reactivity with Bovine, Hamster, Human, Mouse, Rat, and Xenopus laevis samples.
The MA5-13899 immunogen is rat DNA polymerase β protein.
Target Information
DNA polymerase β comprises an amino-terminal 8 kDa domain and a carboxy-terminal 31 kDa domain.
The N-terminal ssDNA binding domain has deoxyribose phosphodiesterase activity, while the C-terminal domain has nucleotidyltransferase activity.
Mammalian DNA polymerase β, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment with DNA-alkylating agents such as MNNG or methyl methanesulfonate up-regulates β-pol levels.
DNA polymerase β fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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