Thermo Fisher Scientific POLB Monoclonal Antibody (18S)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mL

-

ChIP assay (ChIP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Rat, Xenopus

Published species

Human, Mouse, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

18S

Immunogen

Rat DNA polymerase beta protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10977449

Product Specific Information

MA5-13899 targets Rat DNA Polymerase beta in IP and WB applications and shows reactivity with Bovine, Hamster, Human, mouse, Rat, and Xenopus laevis samples.

The MA5-13899 immunogen is rat DNA polymerase beta protein.

Target Information

DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N`-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.