Thermo Fisher Scientific Phospho-alpha Synuclein (Ser129) Recombinant Rabbit Monoclonal Antibody (J18), PerCP
Phospho-alpha Synuclein (Ser129)을 인식하는 재조합 토끼 단클론 항체로 PerCP 결합 형식. Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. 인산화된 Ser129에 특이적이며 비인산화 단백질에는 반응하지 않음. 인간 및 생쥐 시료에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Dot blot (DB) | 1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | J18 |
| Immunogen | Human alpha synuclein AA 124–134: AYEMP-pS-EEGYQ-Cys |
| Conjugate | PerCP |
| Excitation/Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932386 |
Additional Formats
Product Specific Information
A 1:500 dilution of MA5-45932 was sufficient for detection of Alpha Synuclein pSer129 in 10 µg of Mouse Brain by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein.
Target Information
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking.
Alpha- and beta-synuclein selectively inhibit phospholipase D2, indicating involvement in signaling pathways.
Mutations in the SNCA gene, encoding alpha-synuclein, are associated with Parkinson’s disease. Alpha-synuclein peptides are also major components of amyloid plaques in Alzheimer’s disease brains.
Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene.
Diseases associated with SNCA include Parkinson Disease 1 (Autosomal Dominant) and Dementia with Lewy Bodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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