Thermo Fisher Scientific VPS35 Monoclonal Antibody (7E4), APC
VPS35 단백질을 검출하기 위한 Thermo Fisher Scientific의 APC 결합 단일클론 항체. 인간, 마우스, 랫트 반응성. Western blot, ICC/IF, IP에 적합. 고순도 Protein G 정제, 4°C 보관.
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Thermo Fisher Scientific VPS35 Monoclonal Antibody (7E4), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E4 |
| Immunogen | Full length recombinant human VPS35 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932390 |
Additional Formats
- FITC (Cat. No. MA5-45937)
- PerCP (Cat. No. MA5-45938)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45936 detects VPS35 (~92 kDa) in 10 µg of SH-SY5Y lysate by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Target Information
VPS35 is part of a vacuolar protein sorting (VPS) gene family. It encodes a core component of the retromer complex, which mediates retrograde transport of proteins from endosomes to the trans-Golgi network. Structural similarity between yeast and human retromer components suggests conserved function. Expression studies indicate VPS35 interacts directly with other retromer proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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