Thermo Fisher Scientific MCM4 Polyclonal Antibody
Rabbit polyclonal antibody targeting human MCM4 protein. Validated for WB, IHC, IHC-P, and IP applications. Provides high specificity for DNA replication-related protein studies. Supplied as liquid, 1 mg/mL concentration, stored at 4°C.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 750 and 800 of human Minichromosome Maintenance 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) essential for the initiation of eukaryotic genome replication. The hexameric complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may participate in replication fork formation and recruitment of other DNA replication-related proteins.
The MCM complex consisting of MCM2, MCM4, MCM6, and MCM7 exhibits DNA helicase activity and may act as a DNA unwinding enzyme. Phosphorylation by CDC2 kinase reduces the helicase activity and chromatin binding of the complex.
This gene is located on chromosome 8, adjacent to PRKDC/DNA-PK, involved in DNA double-strand break repair. Alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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