Thermo Fisher Scientific mCherry Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 25 publications 25 publications

Immunohistochemistry (IHC)

-

View 18 publications 18 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 8 publications 8 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100-1:500

View 4 publications 4 publications

ChIP assay (ChIP)

-

View 3 publications 3 publications

in situ PLA (PLA)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Mass Spectrometry (MS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length mCherry recombinant protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2552323

Product Specific Information

Recommended positive controls: 293T whole cell extract, human PLK4-mCherry-transfected 293T.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.