
Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eFluor 506, eBioscience
Mouse CD90.2 (Thy-1.2) 단백질을 인식하는 53-2.1 클론의 Rat IgG2a 모노클로날 항체로, eFluor 506 형광체로 결합됨. Flow cytometry에 최적화되어 있으며, 405 nm excitation/506 nm emission 특성. 연구용으로만 사용.
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Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eFluor 506, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 7 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host/Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 506, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-2.1 |
| Conjugate | eFluor™ 506 |
| Excitation/Emission Max | 419/508 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2762803 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 615
- eFluor 450
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 555
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 760
- NovaFluor Red 710
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains (BALB/c, CBA, C3H, C57BL/6, C58/, SJL, etc.). Cells from CD90.1-expressing strains (PL, AKR) do not stain with 53-2.1. CD90 regulates adhesion and signal transduction in T cells.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested by flow cytometric analysis of mouse splenocytes (≤0.5 µg/test). A test is defined as staining a cell sample in 100 µL final volume.
- Recommended: Careful titration for optimal performance.
Fluorochrome properties:
eFluor™ 506 can be excited with a violet laser (405 nm) and emits at 506 nm. Recommended filter: 510/20 band pass or equivalent. Ensure instrument compatibility.
Excitation: 405 nm
Emission: 506 nm
Laser: Violet Laser
Target Information
CD90.2 (Thy-1) antigen is a GPI-linked glycoprotein of the Immunoglobulin superfamily. Expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts. It may play roles in cell-cell interactions during synaptogenesis and other brain events. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons, highly expressed in connective tissue, fibroblast and stromal cell lines, and tumor endothelial cell lines. In mice, CD90.2 is found on all thymocytes and peripheral T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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