Thermo Fisher Scientific GABRA1 Monoclonal Antibody (N95/35), FITC
GABRA1 단백질을 인식하는 FITC 결합 단클론 항체로, WB, IHC, ICC 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성 보유하며, 단백질 G 정제 및 액상 형태로 제공됩니다. 4°C 암소 보관 권장.
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Thermo Fisher Scientific GABRA1 Monoclonal Antibody (N95/35), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N95/35 |
| Immunogen | Fusion protein amino acids 355–394 of human GABA-A-R-Alpha1 |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931940 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45486 was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 55 kDa. No cross-reactivity against GABA-A-R-Alpha2 or -Alpha3.
Target Information
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing hyperpolarization of the membrane through the opening of a Cl⁻ channel associated with the GABAA receptor (GABAA-R) subtype.
GABAA-Rs are key therapeutic targets for sedative, anxiolytic, and hypnotic agents and are implicated in diseases such as epilepsy, anxiety, depression, and substance abuse.
The GABAA-R is a multimeric subunit complex composed of multiple alpha, beta, and gamma subunits, including splice variants. Coexpression of gamma-subunit is required for benzodiazepine modulation, and phosphorylation of beta-subunits can modulate receptor function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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